Diluters for Bovine Semen. I. Fertility and Motility of Bovine Spermatozoa in Boiled Milk

Bovine papillomavirus DNA in milk, blood, urine, semen, and spermatozoa of bovine papillomavirus-infected animals.

Papillomavirus infection in bovines is associated with cutaneous papillomatosis on the hide, udders and other epithelial tissues, as well as in oral respiratory, alimentary and urinary tract mucosa. Bovine papillomavirus (BPV) is also considered the etiological agent of esophageal tumors and the malignant bladder tumors that characterize the clinical condition associated with chronic enzootic h...

The Viability and Fertility of Bovine Spermatozoa

selection of the most appropriate medium for assessing motility and dna uptake of bovine spermatozoa

objective: the aim of this study was to select the best medium to maintain sperm motility during sperm-dna incubation and assess the dna uptake by spermatozoa of iranian holstein bulls and its effects on sperm motility. materials and methods: frozen sperms from an iranian holstein bull were thawed and centrifuged. motile sperms were separated through puresperm gradient (40/80%) followed by two ...

Detection of Bovine Viral Diarrhea Virus in Bovine Semen Using Nested-PCR

A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and nested-PCR were used to detect bovine viral diarrhea virus 1 (BVDV-1) in bull semen. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal...

Glutatione peroxidase in bovine semen.

The roles ofselenium (Se) and glutathione peroxidase in reproductive function are poorly understood, but it is possible that they may be important for normal reproduction in the male. In rats fed a Se-deficient diet, the testes accumulated and retained more 75Se than did other tissues 1 week after injection (Brown & Burk, 1972; Burk, Brown, Seely & Scaief, 1972). Autoradiographic studies (Brown...